Membrane:Article Title: Prostate tumor–derived GDF11 accelerates androgen deprivation therapy–induced sarcopenia
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) ( 48 ), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
Article Title: Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia.
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) (48), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
Incubation:Article Title: Prostate tumor–derived GDF11 accelerates androgen deprivation therapy–induced sarcopenia
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) ( 48 ), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
Article Title: Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia.
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) (48), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
Saline:Article Title: Prostate tumor–derived GDF11 accelerates androgen deprivation therapy–induced sarcopenia
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) ( 48 ), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
Article Title: Prostate tumor-derived GDF11 accelerates androgen deprivation therapy-induced sarcopenia.
Article Snippet: .. The lower membrane was incubated with rat anti-myostatin antibody (1:1000, clone 84214, R&D Systems, Bio-Techne MAB788), and the upper membrane was incubated with rabbit anti-EF2 antibody (1:2000, G270) (48), each diluted in 5% nonfat dried milk in Tris-buffered saline, overnight at 4°C and washed in Tris-buffered saline containing 0.1% Tween-20. .. The lower antimyostatin membrane was incubated in rabbit anti–rat IgG (1:20,000, Pierce, Thermo Fisher Scientific, 21218) for 2 hours at room temperature (RT), and both membranes were incubated in goat anti-rabbit horseradish peroxidase–conjugated antibody (1:20,000, Pierce, Thermo Fisher Scientific, 31460) for 2 hours at RT to detect the rabbit primary and bridging antibodies.
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